游剑——浙江大学药学院教授

游剑，男，汉族，药剂学博士，求是特聘教授，。现任浙江大学药学院药物制剂研究所教授，博士生导师。 

研究领域：药物制剂工程、药物高端制剂、药物临床转化。

主要研究特色：1) 高端药物制剂基础与应用研究，探索药物制剂从基础研究到产业化的关键核心技术，尤其聚焦于脂质体、脂微球等纳米制剂的临床转化。2) 基于先进药物递送技术的免疫与基因治疗。

在国内外重要刊物发表论文200余篇。作为通讯作者，在包括Nat Nanotechnol、Sci Adv、Nat Commun、Adv Sci、Acs nano、ACS Central Sci、J Control Release等发表研究型论文，被他引用5000余次，单篇最高引用800余次。

获发明专利授权20余项，获教育部自然科学奖二等奖、浙江省科学技术奖一等奖两项，获中国药学会青年药剂学奖等。

入选国家万人计划、科技部科技创新领军人才。

教育及工作经历：

 1）大学开始受教育经历 

1994年-1998年，湖南中医药大学，药学院，本科/学士。 

2000年-2005年，沈阳药科大学，药学院，研究生/博士。 

2）研究工作经历 

1998年-2000年，广东丽珠医药集团。 

2005年-2007年，浙江大学药学院，药物制剂研究所，讲师。 

2008年-2011年，美国德克萨斯大学安德森癌症研究中心（University of Texas MD Anderson Cancer Center），实验诊断显像系（Department of Experimental Diagnostic Imaging），博士后。 

2007年-现在，浙江大学药学院，药物制剂研究所，教授、硕士生导师、博士生导师。

主讲课程：

2013-2021 药物制剂专业、本科生专业核心课程：《工业药剂学》、《工业药剂学实验》;

2022年-今 药学专业、本科生专业核心课程：《药剂学》、《药剂学实验》。

培养研究生情况：

指导研究生毕业14名，指导在读研究生19名。

在读研究生：

       主要研究方向：

1）高端药物制剂基础研究：揭示药物制剂体内作用机制、生物学行为、药效评价等

  ◆ 纳米药物及其给药系统

  ◆ 缓控式制剂

  ◆ 长效制剂

  ◆ 疫苗递送

2）药物制剂的临床转化应用研究

探索药物制剂从基础研究到产业化的关键核心技术，尤其是纳米制剂的临床转化

3）药物内质网靶向递送的设计与应用

研究药物靶向递送至细胞内质网的策略以及在肿瘤治疗、免疫疾病治疗、疫苗等方面的应用

4）癌症免疫治疗研究

采用药物制剂手段实现癌症的免疫治疗优化，以及实现多重治疗的结合

5）癌症光热治疗

   以金纳米材料为基础的肿瘤显影技术研究，药物传递研究和靶向肿瘤的光热治疗研究

承担科研项目情况：

1、国家自然科学基金，82574335，靶向骨髓的中央免疫训练与固有免疫记忆持久性关系以及调控疫苗适应性免疫功效研究，2026.1-2029.12

2、国家重点研发计划“前沿生物技术”专项， 2023YFC3403200，mRNA 通用关键技术平台建立及创新药物研发，课题组长， 2024.1-2028.12

3、国家自然科学基金，82273862，抗病毒疫苗精准调控机体细胞与体液免疫策略及其机制研究，2023.1-2026.12

4、重大研究横向项目（1500万），创新高端制剂研发平台， 2022-2027

5、浙江省公益项目，LGF22H300003，调控肿瘤相关巨噬细胞内质网应激影响其分化以增强ICB效果研究，2022-2024

6、国家“万人计划”人才项目，2020.12-2023.12

7、重大研究横向项目（750万），难溶性药物创新制剂产品开发， 2020-2025

8、国家自然科学基金，81973246，内质网靶向近红外光动力效应调控肿瘤细胞免疫原性死亡机制及在免疫治疗中的应用，2020.1-2023.12

9、浙江省基础公益研究计划，LGF18H300004，改善肿瘤缺氧针对癌症转移的双向免疫治疗及其机制研究，2018.1-2019.12

10、国家自然科学基金，81573365，针对中晚期癌症的光热免疫治疗纳米系统的构建与作用机制研究，2016.1-2019.12

11、重大研究横向项目（1000万），浙江大学-索安克新药研发平台， 2015-2020

12、国家自然科学基金，81373348，基于金纳米粒给药系统的近红外光介导同步光热与光动力治疗基础研究，2014.1-2017.12

13、浙江省钱江人才计划，2013R10043，新型核壳结构金纳米材料用于肿瘤的近红外光热治疗研究，2013.07-2015.06 

14、国家自然科学基金，81001411，Ephrin多肽介导的多功能中空金纳米壳-聚合物胶束作为肿瘤靶向治疗载体的研究，2011.1-2013.12。 

15、国家973计划子课题，2009CB930305，纳米技术改善药物功效的生物安全性研究，2009.1-2013.12，子课题负责人。

16、浙江省科技厅项目，2008C23043，叶酸修饰糖脂结构聚合物胶团肿瘤靶向性研究， 2008.1-2010.12。  

参与科研项目： 

1. 国家自然科学基金面上项目，50573066，智能性环糊精高分子纳米囊研究，2006.1-2006.12，8万，排名2/8，已完成。 

2. 国家自然科学基金面上项目，30672552，细胞核靶向载体的设计与抗肿瘤药效学研究，2007.1-2009.12，25万，排名3/7，已完成。 

3. 国家863计划课题，2007AA03Z318，靶向药物分子靶区纳米材料制备与应用， 2007.12-2010.11，100万，排名3/12，已完成。 

4. 国家自然科学基金面上项目，30973683，基于难溶性药物结构的聚合物胶束载体分子设计及其特征研究，2010.1-2012.12，25万，排名2/8，正在进行。

科研成果：

1． “脂质纳米载体给药系统的基础理论研究”2010年获国家自然科学二等奖。

2． 细胞浆靶向壳寡糖-硬脂酸嫁接物纳米载体材料的制备与应用 胡富强; 袁弘; 杜永忠; 游剑; 叶邦阜; 万丽卿; 叶轶青; 赵梦丹; 缪静 【科技成果】浙江大学药学院 2007-02-01

3． 抗肿瘤靶向脂质纳米给药系统的制备与应用 袁弘; 杜永忠; 胡富强; 游剑; 应晓英; 叶轶青; 黄凌斐 【科技成果】浙江大学 2008-07-13

发明公开：

[1]罗利华, 游剑, 张俊镭, 胡义龙, 毛佳鹏, 朱淳琪. 基于最佳给药顺序优化的药物控释系统及其制备方法和应用[P]. 浙江省: CN120585729A, 2025-09-05.

[2]杨富春, 游剑, 罗利华, 胡义龙, 柴秦明, 孙飞. 多细胞稳态靶向调控的肝纤维化治疗纳米组合物的制备及应用[P]. 浙江省: CN120437057A, 2025-08-08.

[3]罗利华, 游剑, 周欢丽, 杨富春, 朱淳琪. 基于细胞稳态调控逆转肿瘤化疗抵抗的纳米组合物制备及应用[P]. 浙江省: CN120437058A, 2025-08-08.

[4]罗琼, 游剑, 蒋颖, 陈原, 李青坡. 一种具有免疫微环境调节功能的疤痕修复贴及其应用[P]. 浙江省: CN120360973A, 2025-07-25.

[5]游剑, 张俊镭, 黄嘉信. 基于脂质液晶技术的长效镇痛组合物及应用[P]. 浙江省: CN119280165A, 2025-01-10.

[6]游剑, 黄嘉信. 一种高效包封的pH响应性载药脂质体制备方法及应用[P]. 浙江省: CN119280169A, 2025-01-10.

[7]游剑, 罗利华, 张玉玮, 王丽彤, 张一涛. 一种载细菌全抗原的PLGA微球疫苗及其制备方法与应用[P]. 浙江省: CN119185522A, 2024-12-27.

[8]游剑, 程宁涛, 罗震宇, 蒋梦诗, 罗利华. 一种肝脏特异性靶向纳米制剂和应用[P]. 浙江省: CN118750445A, 2024-10-11.

[9]游剑, 楼泽亮, 施莹莹. 一种基于佐剂-抗原时序控制的mRNA疫苗构建及其应用[P]. 浙江省: CN118649232A, 2024-09-17.

[10]游剑, 施莹莹. 一种环境适应性协同调控的mRNA纳米递送系统的构建与应用[P]. 浙江省: CN118267481A, 2024-07-02.

[11]游剑, 单新禹. 一种长效脂质液晶植入物及其治疗阿尔茨海默症的用途[P]. 浙江省: CN117797095A, 2024-04-02.

[12]游剑, 陆益超. 一种缓解适应性皮炎的还原性佐剂纳米系统及应用[P]. 浙江省: CN116850279A, 2023-10-10.

[13]游剑, 郭雪萌. 一种具有抗氧化功能的纳米制剂及治疗青光眼用途[P]. 浙江省: CN116725954A, 2023-09-12.

[14]游剑, 赵小奇, 张俊镭. 超高包封率的重酒石酸长春瑞滨脂质微球及制备与应用[P]. 浙江省: CN116725963A, 2023-09-12.

[15]游剑, 郭雪萌, 石美幸. 一种包载他克莫司的纳米制剂及应用[P]. 浙江省: CN116672312A, 2023-09-01.

[16]游剑, 罗震宇. 一种基于中性粒细胞的骨髓靶向给药载体及应用[P]. 浙江省: CN116459351A, 2023-07-21.

[17]游剑, 施莹莹, 楼泽亮. 靶向清除抗原特异性T细胞的核壳结构纳米粒构建与应用[P]. 浙江省: CN116115772A, 2023-05-16.

[18]游剑, 林晴, 李青坡. 一种释放可控的曲安奈德植入物及其用于防治关节炎用途[P]. 浙江省: CN116077427A, 2023-05-09.

[19]游剑, 施莹莹. 均衡诱导抗病毒细胞与体液免疫的核酸-纳米乳的构建与应用[P]. 浙江省: CN115444931A, 2022-12-09.

[20]游剑, 罗利华, 李青坡. 一种载病毒抗原和佐剂的多级靶向载体的构建及应用[P]. 浙江省: CN114939159A, 2022-08-26.

[21]游剑, 刘旭, 黄嘉信. 一种用于装载水溶性药物的脂质体制备方法[P]. 浙江省: CN114869851A, 2022-08-09.

[22]游剑, 张俊镭. 一种精氨酸多囊脂质体的制备方法[P]. 浙江省: CN114848591A, 2022-08-05.

[23]游剑, 张俊镭, 李青坡. 靶向免疫细胞长效补充精氨酸并中和酸环境的组合物及应用[P]. 浙江省: CN114788870A, 2022-07-26.

[24]游剑, 陆益超, 罗震宇, 李青坡. 用于局部和全身性减脂的纳米制剂及其应用[P]. 浙江省: CN114712514A, 2022-07-08.

[25]游剑, 施莹莹, 李青坡. 一种可逆转酒精性肝病的维生素E纳米载体的构建与应用[P]. 浙江省: CN114469861A, 2022-05-13.

[26]游剑, 罗震宇. 一种能双向连接磁性纳米粒的构建方法与应用[P]. 浙江省: CN113648426A, 2021-11-16.

[27]游剑, 陆益超, 李青坡, 姜新东. 恢复肿瘤微环境失活树突状细胞功能的纳米制剂及应用[P]. 浙江省: CN113546045A, 2021-10-26.

[28]游剑, 施莹莹, 李青坡, 姜新东. 调控适应性细胞与体液免疫的纳米载体的构建与应用[P]. 浙江省: CN113171450A, 2021-07-27.

[29]游剑, 刘雨. 一类细胞内质网靶向纳米载药系统的构建与应用[P]. 浙江省: CN112472822A, 2021-03-12.

[30]游剑, 罗利华. 靶向至细胞内质网纳米载药系统的构建与应用[P]. 浙江省: CN109432432A, 2019-03-08.

[31]游剑, 裘云庆, 楼燕, 郭晓萌. 一种增敏肿瘤放疗的纳米脂质体及应用[P]. 浙江: CN109045284A, 2018-12-21.

[32]游剑, 曲佳鑫. 一种携氧增强光动力的光敏脂质体及制备与应用[P]. 浙江: CN105997877A, 2016-10-12.

[33]游剑, 张汉波. 一种透明质酸维生素E衍生物及制备和应用[P]. 浙江: CN104945538A, 2015-09-30.

[34]杜永忠, 司徒俊青, 余日胜, 应晓英, 游剑, 赵梦丹. A54-葡聚糖-聚乳酸-羟基乙酸共聚物及制备与应用[P]. 浙江: CN104311831A, 2015-01-28.

[35]游剑, 周佳林, 胡富强, 杜永忠, 袁弘. 一种增强光热转化效应的核壳金纳米粒[P]. 浙江: CN104147601A, 2014-11-19.

[36]胡富强, 吴晖, 袁弘, 姚忠立, 欧阳亚华, 刘峻, 杜永忠, 游剑. 一种稳定的软胶囊囊壳及其制备方法[P]. 浙江: CN103800912A, 2014-05-21.

[37]胡富强, 吴晖, 袁弘, 陈眉眉, 姚忠立, 李海杰, 杜永忠, 游剑, 赵川平. 一种泮托拉唑钠药物组合物、含有该组合物的微丸及其制备方法[P]. 浙江: CN103800326A, 2014-05-21.

[38]胡富强, 吴晖, 袁弘, 欧阳亚华, 韩敏, 陈眉眉, 李海杰, 杜永忠, 游剑, 赵川平. 一种水性肠溶包衣液及其制备方法[P]. 浙江: CN103784964A, 2014-05-14.

[39]游剑, 张培尊, 胡富强, 杜永忠, 袁弘. 一种包载水溶性药物的光敏脂质体[P]. 浙江: CN103284951A, 2013-09-11.

[40]胡富强, 胡颖文, 杜永忠, 袁弘, 游剑. 一种谷胱甘肽敏感性烷基化壳聚糖及制备与应用[P]. 浙江: CN102875695A, 2013-01-16.

[41]虞和永, 游剑, 李云龙, 胡富强, 袁弘, 杜永忠. 一种具抗肿瘤活性的紫杉醇脂质纳米粒注射液[P]. 浙江: CN102871963A, 2013-01-16.

[42]胡富强, 杜永忠, 袁弘, 游剑. 叶酸修饰的壳寡糖-硬脂酸嫁接物及制备方法和应用[P]. 浙江: CN101148483, 2008-03-26.

[43]胡富强, 杜永忠, 袁弘, 游剑. 一种纳米结构脂质载体给药系统的用途[P]. 浙江: CN101129335, 2008-02-27.

[44]袁弘, 杜永忠, 胡富强, 游剑. 一种脂质纳米粒的制备方法[P]. 浙江: CN101129336, 2008-02-27.

[45]游剑, 胡富强, 袁弘, 杜永忠. 长春瑞滨固体脂质纳米粒与其冻干制剂及制备方法[P]. 浙江: CN101129375, 2008-02-27.

[46]胡富强, 杜永忠, 袁弘, 游剑. 叶酸烷基化衍生物及其应用[P]. 浙江: CN101092416, 2007-12-26.

[47]胡富强, 杜永忠, 袁弘, 游剑. 亚细胞器靶向壳寡糖-脂肪酸嫁接物及制备和应用[P]. 浙江: CN101081876, 2007-12-05.

[48]胡富强, 杜永忠, 袁弘, 游剑. 可逆转肿瘤细胞耐药性的细胞核靶向给药系统的应用[P]. 浙江: CN101066458, 2007-11-07.

[49]胡富强, 杜永忠, 袁弘, 游剑. 细胞核靶向壳寡糖-脂肪酸嫁接物载药胶团的应用[P]. 浙江: CN101066461, 2007-11-07.

[50]胡富强, 杜永忠, 袁弘, 游剑. 可逆转肿瘤细胞耐药性的细胞浆靶向给药系统的应用[P]. 浙江: CN101066462, 2007-11-07.

[51]胡富强, 杜永忠, 袁弘, 游剑. 一种具有高效抗肿瘤活性的纳米结构脂质载体[P]. 浙江: CN101011358, 2007-08-08.

[52]胡富强, 杜永忠, 袁弘, 游剑. 一种具有抗肿瘤活性的固体脂质纳米粒[P]. 浙江: CN101011359, 2007-08-08.

[53]胡富强, 杜永忠, 袁弘, 游剑. 细胞浆靶向壳寡糖-脂肪酸嫁接物载药胶团的应用[P]. 浙江: CN101011579, 2007-08-08.

[54]胡富强, 杜永忠, 袁弘, 游剑. 异硫氰基荧光素硬脂胺嫁接物及其合成方法和应用[P]. 浙江: CN101012231, 2007-08-08.

[55]胡富强, 杜永忠, 袁弘, 游剑, 任国飞. 表面修饰疏水改性壳寡糖聚合物载药胶团及其制备方法[P]. 浙江: CN1883708, 2006-12-27.

[56]胡富强, 杜永忠, 袁弘, 游剑, 应晓英, 赵梦丹. 一种非病毒基因转染载体及制备方法和用途[P]. 浙江: CN1844402, 2006-10-11.

[57]胡富强, 袁弘, 应晓英, 杜永忠, 游剑. 一种难溶性药物纳米粒及制备方法[P]. 浙江: CN1771910, 2006-05-17.

[58]胡富强, 袁弘, 应晓英, 杜永忠, 游剑, 田治科. 一种可再分散的难溶性药物纳米粒粉末及制备方法[P]. 浙江: CN1771911, 2006-05-17.

[59]崔福德, 李青坡, 游剑. 酶降解依赖型结肠定位释药柱塞胶囊及其制备方法[P]. 辽宁: CN1582903, 2005-02-23.

[60]崔福德, 游剑. 液相中制备液体油性药物的高包油型固体微丸及其制备方法[P]. 辽宁: CN1511510, 2004-07-14. 

发明授权：

[1]游剑, 罗震宇. 一种基于中性粒细胞的骨髓靶向给药载体及应用[P]. 浙江省: CN116459351B, 2025-06-27.

[2]游剑, 施莹莹, 楼泽亮. 靶向清除抗原特异性T细胞的核壳结构纳米粒构建与应用[P]. 浙江省: CN116115772B, 2025-06-17.

[3]游剑, 罗震宇. 一种能双向连接磁性纳米粒的构建方法与应用[P]. 浙江省: CN113648426B, 2024-10-29.

[4]游剑, 张俊镭, 李青坡. 靶向免疫细胞长效补充精氨酸并中和酸环境的组合物及应用[P]. 浙江省: CN114788870B, 2023-06-06.

[5]游剑, 陆益超, 罗震宇, 李青坡. 用于局部和全身性减脂的纳米制剂及其应用[P]. 浙江省: CN114712514B, 2023-03-31.

[6]游剑, 刘旭, 黄嘉信. 一种用于装载水溶性药物的脂质体制备方法[P]. 浙江省: CN114869851B, 2023-03-28.

[7]游剑, 施莹莹, 李青坡. 一种可逆转酒精性肝病的维生素E纳米载体的构建与应用[P]. 浙江省: CN114469861B, 2023-02-03.

[8]游剑, 陆益超, 李青坡, 姜新东. 恢复肿瘤微环境失活树突状细胞功能的纳米制剂及应用[P]. 浙江省: CN113546045B, 2022-11-11.

[9]游剑, 刘雨. 一类细胞内质网靶向纳米载药系统的构建与应用[P]. 浙江省: CN112472822B, 2022-05-27.

[10]游剑, 罗利华. 靶向至细胞内质网纳米载药系统的构建与应用[P]. 浙江省: CN109432432B, 2020-10-23.

[11]游剑, 周佳林, 胡富强, 杜永忠, 袁弘. 一种增强光热转化效应的核壳金纳米粒[P]. 浙江省: CN104147601B, 2017-07-25.

[12]游剑, 张培尊, 胡富强, 杜永忠, 袁弘. 一种包载水溶性药物的光敏脂质体[P]. 浙江省: CN103284951B, 2015-12-23.

[13]胡富强, 吴晖, 袁弘, 姚忠立, 欧阳亚华, 刘峻, 杜永忠, 游剑. 一种稳定的软胶囊囊壳及其制备方法[P]. 浙江省: CN103800912B, 2015-12-09.

[14]胡富强, 吴晖, 袁弘, 欧阳亚华, 韩敏, 陈眉眉, 李海杰, 杜永忠, 游剑, 赵川平. 一种水性肠溶包衣液及其制备方法[P]. 浙江省: CN103784964B, 2015-06-17.

[15]胡富强, 吴晖, 袁弘, 陈眉眉, 姚忠立, 李海杰, 杜永忠, 游剑, 赵川平. 一种泮托拉唑钠药物组合物、含有该组合物的微丸及其制备方法[P]. 浙江省: CN103800326B, 2015-06-17.

[16]游剑, 胡富强, 袁弘, 杜永忠. 长春瑞滨固体脂质纳米粒与其冻干制剂及制备方法[P]. 浙江省: CN101129375B, 2010-12-22.

[17]胡富强, 杜永忠, 袁弘, 游剑. 一种纳米结构脂质载体给药系统的用途[P]. 浙江省: CN101129335B, 2010-09-22.

[18]胡富强, 杜永忠, 袁弘, 游剑. 细胞浆靶向壳寡糖-脂肪酸嫁接物载药胶团的应用[P]. 浙江省: CN101011579B, 2010-09-08.

[19]胡富强, 杜永忠, 袁弘, 游剑. 可逆转肿瘤细胞耐药性的细胞核靶向给药系统的应用[P]. 浙江省: CN101066458B, 2010-05-26.

[20]胡富强, 袁弘, 应晓英, 杜永忠, 游剑, 田治科. 一种可再分散的难溶性药物纳米粒粉末及制备方法[P]. 浙江省: CN1771911B, 2010-05-12.

[21]崔福德, 游剑. 液相中制备液体油性药物的高包油型固体微丸及其制备方法[P]. 辽宁省: CN1511510B, 2010-04-28.

[22]袁弘, 杜永忠, 胡富强, 游剑. 一种脂质纳米粒的制备方法[P]. 浙江省: CN101129336B, 2010-04-14.

[23]胡富强, 杜永忠, 袁弘, 游剑. 叶酸修饰的壳寡糖－硬脂酸嫁接物及制备方法和应用[P]. 浙江省: CN101148483B, 2010-04-07.

[24]胡富强, 杜永忠, 袁弘, 游剑. 亚细胞器靶向壳寡糖－脂肪酸嫁接物的合成方法[P]. 浙江省: CN100569802C, 2009-12-16.

[25]胡富强, 杜永忠, 袁弘, 游剑. 细胞核靶向壳寡糖－脂肪酸嫁接物载药胶团的应用[P]. 浙江省: CN100562341C, 2009-11-25.

[26]胡富强, 杜永忠, 袁弘, 游剑. 叶酸烷基化衍生物及其应用[P]. 浙江省: CN100560586C, 2009-11-18.

[27]胡富强, 杜永忠, 袁弘, 游剑. 可逆转肿瘤细胞耐药性的细胞浆靶向给药系统的应用[P]. 浙江省: CN100525837C, 2009-08-12.

[28]胡富强, 杜永忠, 袁弘, 游剑. 一种具有抗肿瘤活性的固体脂质纳米粒[P]. 浙江省: CN100515391C, 2009-07-22.

[29]胡富强, 杜永忠, 袁弘, 游剑. 一种具有高效抗肿瘤活性的纳米结构脂质载体[P]. 浙江省: CN100479806C, 2009-04-22.

[30]胡富强, 杜永忠, 袁弘, 游剑, 任国飞. 表面修饰疏水改性壳寡糖聚合物载药胶团及其制备方法[P]. 浙江省: CN100417417C, 2008-09-10.

发表英文论文： 

[1]Litong Wang, Yitao Zhang, Shenyu Wang, Xin Shen, Junlei Zhang, Jian You*, Lihua Luo*. Nano-strategy empowering trained immunity in vaccines. J Control Release 2025, 114359. (IF: 10.5)

[2]Junlei Zhang, Sijie Wang, Huihui Liu, Lihua Luo, Jian You*. Arginine Competition in Tumor Microenvironment: A Potential Target for Cancer Therapy. Med Res Rev 2025, DOI: 10.1002/med.70015. (IF: 11.6)

[3]Huihui Liu, Yilong Hu, Wanting Ji, Sijie Wang, Ying Zhu, Qing Lin, Xiaoqi Zhao, Huanli Zhou, Xuemeng Guo, Yu Liu, Yingying Shi, Junlei Zhang, Zhenyu Luo, Jiaxin Huang, Hang Yin, Lihua Luo, Jian You*, Sustained Activation of Nrf2 Antioxidant Pathway by Flexible Liposome Based on Low Phase Transition Temperature to Delay Skin Aging. Adv Healthcare Mater 2025, In press. (IF:10.08)

[4]Yingying Shi, Kedong Sun, Yilong Hu, Zeliang Lou, Yi Wang, Jian You*, The Strategies and Advances of mRNA Translation Booster. AJPS 2025, 101090 (IF:10.2)

[5]Sijie Wang, Huanli Zhou, Jiapeng Mao, Yitao Zhang, Yuxin Qi, Mei Pang, Xizhi Jin, Junlei Zhang, Lihua Luo, Jian You*, Precision nanomedicine for pneumonocyte-targeting: Emerging strategies and clinical prospects in refractory pulmonary disease therapy, Biomaterials 2025, 323, 123420.  (IF 12.8)

[6]Xu Liu, Shuhan Ji, Yichen Cai, Fuchun Yang, Jian You*, Lihua Luo*. Advances in mRNA-LNP lung-targeted delivery strategies. Microstructures 2025, 5. 

[7]Yingying Shi, Zeliang Lou, Yingchao Wang, Min Li, Yi Wang, Jian You*. Iterative Upgrading of mRNA Therapeutics by Modulating the Microenvironment for Translation. Advanced Functional Materials 2025, 2414722. (IF 19.924)

[8]Litong Wang, Yitao Zhang, Jiaxin Huang, Sijie Wang, Shuhan Ji, Shenyu Wang, Meixing Shi, Junlei Zhang, Yingying Shi, Zhenyu Luo, Zhaolei Jin, Xindong Jiang, Qingpo Li, Fuchun Yang*, Jian You*, Lihua Luo*. Vaccine Specifically for Immunocompromised Individuals against Superbugs. ACS nano 2025, 19, 3372-3391 doi.org/10.1021/acsnano.4c12203. (IF: 15.8)

[9]Lu, Yichao; Shan, Xinyu; Huang, Jiaxin; Zhou, Huanli; Zhu, Ying; Luo, Zhenyu; Liu, Xu; Wang, Sijie; Guo, Xuemeng; Shi, Yingying; Hu, Yilong; Liu, Huihui; Zhang, Junlei; Li, Qingpo; Luo, Lihua; You, Jian*. Reductive Adjuvant Nano-system for Alleviated Atopic Dermatitis Syndromes. ACS Nano 2025, 19, 4195-4212. (IF: 15.8)

[10]Xu Liu, Jiaxin Huang , Huanli Zhou, Sijie Wang, Xuemeng Guo, Jiapeng Mao, Xiang Li, Yichao Lu, Yongzhong Du, Fuchun Yang, Lihua Luo, Jian You*. Inhibition of PDT-induced PGE2 surge for enhanced photo-immunotherapy, Biomaterials 2025, 123116. (IF 12.8)

[11]Yingying Shi, Jiapeng Mao, Jian You*, Pharmaceutical Strategies for Optimized mRNA Expression. Biomaterials 2024. 314, 122853. (IF 12.8)

[12]Xuemeng Guo, Xiang Li, Sijie Wang, Yingying Shi, Jiaxin Huang, Xu Liu, Yichao Lu, Junlei Zhang, Lihua Luo, Jian You*, Optimizing Adoptive Cell Therapy for Solid Tumors via Epigenetic Regulation of T Cell Destiny, Adv Healthcare Mater 2024, 2402209. (IF:10.08)

[13]Yingying Shi, Meixing Shi, Yi Wang, Jian You*, Progress and prospects of mRNA-based drugs in pre-clinical and clinical applications, Signal Transduction and Targeted Therapy 2024, 9, 322. (IF: 40.88)

[14]Zhenyu Luo, Mengshi Jiang, Ningtao Cheng, Xiaoqi Zhao, Huihui Liu, Sijie Wang, Qing Lin, Jiaxin Huang, Xuemeng Guo, Xu Liu, Xinyu Shan, Yichao Lu, Yingying Shi, Lihua Luo, Jian You*. Remodeling the hepatic immune microenvironment and demolishing T cell traps to enhance immunotherapy efficacy in liver metastasis. J Control Release 2024, 373, 890-904. (IF: 10.5)

[15]Zeliang Lou, Yingying Shi, Xuemeng Guo, Zhaolei Jin, Jiaxin Huang, Yilong Hu, Xu Liu, Jiang Zhu, Rong Kuang, and Jian You*. Chronological management of adjuvant effect for optimized mRNA vaccine inspired by natural virus infection. ACS Nano 2024, 18, 19694-19711. (IF: 15.8)

[16]Ziyao Huang, Kedong Sun, Zhenyu Luo, Junlei Zhang, Huanli Zhou, Hang Yin, Zhile Liang, Jian You*. Spleen-targeted delivery systems and strategies for spleen-related diseases. J Control Release 2024, 370, 773-797. (IF: 10.5)

[17]Shan, Xinyu; Lu, Yichao; Luo, Zhenyu; Zhao, Xiaoqi; Pang, Mei; Yin, Hang; Guo, Xuemeng; Zhou, Huanli; Zhang, Junlei ; Huang, Jiaxin; Shi, Yingying ; Lou, Jinfang; Luo, Lihua; You, Jian*. A long-acting lyotropic liquid crystalline implant promotes the drainage of macromolecules by brain-related lymphatic system in treating aged Alzheimer's disease. ACS Nano 2024, 18, 9688-9703. (IF: 15.8)

[18]Yichao Lu, Xu Liu, Jian You*. Mechanisms and measures to modulate T cell trafficking for amplified and tolerogenic immunity, Nano Today 2024, 54, 102129. (IF: 18.962)

[19]Xu Liu, Yichao Lu, Xiang Li, Lihua Luo, Jian You*. Recent progress in nanoplatform-enhanced photodynamic therapy for the induction of immunogenic cell death. J Control Release 2024, 365, 1058-1073 (IF: 11.467)

[20]Yichao Lu, Zhenyu Luo, Huanli Zhou, Yingying Shi, Ying Zhu, Xuemeng Guo, Jiaxin Huang, Junlei Zhang, Xu Liu, Sijie Wang, Xinyu Shan, Hang Yin, Yongzhong Du, Qingpo Li, Jian You*, Lihua Luo*. A Nanoemulsion Targeting Adipose Hypertrophy and Hyperplasia Shows Anti-Obesity Efficiency in Female Mice. Nature Communications 2024, 15, 72.  (IF: 16.6)

[21]Luo, Lihua; Wang, Sijie; Hu, Yilong; Wang, Litong; Jiang, Xindong; Zhang, Junlei; Liu, Xu; Guo, Xuemeng; Luo, Zhenyu; Zhu, Chunqi; Xie, Miaomiao; Li, Yeqing; You, Jian*; Yang, Fuchun. Precisely Regulating M2 Subtype Macrophages for Renal Fibrosis Resolution. ACS nano 2023, 17, 22508-22526. (IF: 18.027).

[22]Xu Liu, Junlei Zhang, Xuemeng Guo, Jiaxin Huang, Zeliang Lou, Xiaoqi Zhao, Qing Lin, Xiang Li, Jian You*, and Lihua Luo, Enhancing Tumor Immunotherapy via Photodynamic Therapy with a Cascade Reaction of Reactive Oxygen Species and Sustaining Nutrient Supply. J Control Release 2023, 364, 343-356 (IF: 11.467)

[23]Lihua Luo, Zhenyu Luo, Litong Wang, Yilong Hu, Junlei Zhang, Hang Yin, Jian You*, Liposome Vaccine for Active Regulation of Cellular and Humoral Immunity, Mol Pharm 2023, In press (IF: 4.9)

[24]Qin Lin, Xinyu Shan, Xiang Li, Zhenyu Luo, Xin Yu, Huihui Liu, Sijie Wang, Xiaoqi Zhao, Ying Zhu, Huanli Zhou, Lihua Luo, Jian You*. Solvent Exchange-Motivated and Tunable in Situ Forming Implants Sustaining Triamcinolone Acetonide Release for Arthritis Treatment. Int J Pharmaceut 2023, 123383.(IF: 5.875)

[25]Yingying Shi, Zeliang Lou, Yu Liu, Jiaxin Huang, Yi Wang, Jian You*. Cell communication-mediated selective drug delivery for epitope-specific deletion of alloreactive T cells in organ transplant. Advanced Functional Materials 2023, 2304823. (IF: 19.924)

[26]Xuemeng Guo, Junlei Zhang, Xu Liu, Yichao Lu, Yingying Shi, Xiang Li, Sije Wang, Jiaxin Huang, Huihui Liu, Huanli Zhou, Qingpo Li, Lihua Luo, Jian You*. Antioxidant nanoemulsion loaded with latanoprost enables highly effective glaucoma treatment. J Control Release 2023, 361, 534-546. (IF: 11.467)

[27]Xiaoqi Zhao, Xuemeng Guo, Mei Pang, Weigen Qiu, Zhenyu Luo, Qing Lin, Yichao Lu, Hang Yin, Sijie Wang, Huihui Liu, Junlei Zhang, Lihua Luo, Jian You*. Reductive lipid nanoparticles loaded with vinorelbine inhibit chemotherapy-induced invasion of cancer cells by modulating ENPP2. Nano Research 2023, doi.org/10.1007/s12274-023-6030-2 (IF: 10.269)

[28]Sijie Wang, Junlei Zhang, Huanli Zhou, Yi Chao Lu, Xizhi Jin, LihuaLuo, JianYou*. The role of protein corona on nanodrugs for organ-targeting and its prospects of application. J Control Release 2023, 360, 15-43. (IF: 11.467)

[29]Sijie Wang, Junlei Zhang, Huihui Liu, Xuemeng Guo, Yichao Lu, Guannan Guan, Zhenyu Luo, Mengshi Jiang, Qing Lin, Huanli Zhou, Xiaoqi Zhao, Lihua Luo, Jian You*. The Effect of Blood Velocity in Solid Tumor on Intratumorally Accumulation and Penetration of Nanocarriers and Drugs. Nano Today 2023, 50, 101870. (IF: 18.962)

[30]Lihua Luo, Huanli Zhou, Sijie Wang, Mei Pang, Junlei Zhang, Yilong Hu, Jian You*, The application of nanoparticle-based imaging and phototherapy for female reproductive organs diseases, Small 2023, 2207694.  (IF: 15.153)

[31]Zhenyu Luo, Yichao Lu, Yingying Shi, Mengshi Jiang, Xinyu Shan, Xiang Li, Junlei Zhang, Bing Qin, Xu Liu, Xuemeng Guo, Jiaxin Huang, Yu Liu, Sijie Wang, Qingpo Li, Lihua Luo, Jian You* . Neutrophil Hitchhiking for Drug Delivery to the Bone Marrow. Nature Nanotechnology 2023, 18, 647-656.(IF: 40.523)

[32]Shan, Xinyu; Li, Xiang ; Luo, Zhenyu; Lin, Qing; Lu, Yichao; Jiang, Mengshi; Zhang, Junlei ; Huang, Jiaxin; Xie, Lin ; Guo, Xuemeng; Liu, Xu; Shi, Yingying ; Liu, Yu; Yin, Hang; Yang, Fuchun; Luo, Lihua; You, Jian*. A clinically-achievable injectable and sprayable in-situ lyotropic liquid crystalline platform in treating hormone-sensitive and castration-resistant prostate cancer. ACS nano 2023, 17, 6045-6061.(IF: 18.027)

[33]Xiang Li, Xuemeng Guo, Jiaxin Huang, Qing Lin, Bing Qin, Mengshi Jiang, Xinyu Shan, Zhenyu Luo, Junlei Zhang, Yingying Shi, Yichao Lu, Xu Liu, Yongzhong Du, Fuchun Yang, Lihua Luo, Jian You*, Recruiting T cells and sensitizing tumors to NKG2D immune surveillance for robust antitumor immune response, J Control Release 2023, 353, 943-955. (IF: 11.467)

[34]Lihua Luo, Zhenyu Luo, Junlei Zhang, Xu Liu, Jiaxin Huang, Sijie Wang, Hang Yin, Xuemeng Guo, Yilong Hu, Yichao Lu, Xinyu Shan, Huihui Liu, Yingying Shi, Yongzhong Du, Fuchun Yang, Jian You*. Preventing Acute Lung Injury from Progressing to Pulmonary Fibrosis by Maintaining ERS Homeostasis Through a Multistage Targeting Nanomicelle. Nano Today 2022, 48，101719. (IF: 18.962)

[35]Yu Liu, Xu Liu, Jiaxin Huang, Yingying Shi, Zhenyu Luo, Junlei Zhang, Xuemeng Guo, Mengshi Jiang, Xiang Li, Hang Yin, Bing Qin, Guannan Guan, Lihua Luo, Yun Zhou, Jian You*. Non-lysosomal Route of mRNA delivery and Combining with Epigenetic Regulation Optimized antitumor Immunoprophylactic Efficacy. Advanced Healthcare Materials 2022, 11, e2202460. (IF: 11.092)

[36]Yingying Shi, Jiaxin Huang, Yu Liu, Jing Liu, Xuemeng Guo, Jianhua Li, Liming Gong, Xin Zhou, Guofeng Cheng, Yunqing Qiu, Jian You*,  Yan Lou*. Structural and biochemical characteristics of mRNA nanoparticles determine anti-SARS-CoV-2 humoral and cellular immune responses, Science Advances 2022, 8, eabo1827. (IF: 14.96)

[37]Jun Meng, Zhixin Yu, Hongying Chen, Xin Yu, Mengshi Jiang, Xin-An Zeng, Jian You*, Brucea javanica oil emulsion significantly improved the effect of anti-programmed cell death protein-1 immunotherapy, Phytomedicine 2022, 154446 (IF: 6.65)

[38]Zhang, Junlei ; Wang, Sijie; Guo, Xuemeng; Lu, Yichao; Liu, Xu; Jiang, Mengshi; Li, Xiang ; Qin, Bing; Luo, Zhenyu; Liu, Huihui; Li, Qingpo; Du, Yong-Zhong; Luo, Lihua; You, Jian*. Arginine supplementation targeting tumor-killing immune cells reconstructs tumor microenvironment and enhances antitumor immune response. ACS nano 2022, 16, 12964-12978.  (IF: 18.027)

[39]Yingying Shi, Yu Liu, Sijie Wang, Jiaxin Huang, Zhenyu Luo, Mengshi Jiang, Yichao Lu, Qing Lin, Huihui Liu, Ningtao Cheng, Jian You*, Endoplasmic reticulum-targeted inhibition of CYP2E1 with Vitamin E nanoemulsions alleviates hepatocyte oxidative stress and reverses alcoholic liver disease, Biomaterials 2022, 121720.(IF: 15.304)

[40]Yingying Shi, Yichao Lu, Jian You*, Antigen transfer and its effect on vaccine-induced immune amplification and tolerance, Theranostics 2022, 12, 5888-5913.(IF: 11.6)

[41]Mengshi Jiang, Bing Qin, Xiang Li, Yu Liu, Guannan Guan, Jian You*, New Advances in Pharmaceutical Strategies for Sensitizing Anti-PD-1 Immunotherapy and Clinical Research, WIREs Nanomedicine & Nanobiotechnology 2022, e1837.(IF: 9.42)

[42]Yichao Lu, Yingying Shi, Yu Liu, Zhenyu Luo, Junlei Zhang, Mengshi Jiang, Xiang Li, Xu Liu，Xuemeng Guo, Bing Qin, Hang Yin, Yongzhong Du, Yunqing Qiu, Yan Lou, Guannan Guan, Lihua Luo, Jian You*, A Therapeutic DC Vaccine with Maintained Immunological Activity Exhibits Robust Anti-Tumor Efficacy, J Control Release 2022, 349, 254-268. (IF: 11.467)

[43]Xinyu Shan, Lihua Luo, Zhixin Yu, Xiang Li, Mengshi Jiang, Jian You*, Recent advances in versatile inverse lyotropic liquid crystals. J Control Release 2022, 348, 1-21. (IF: 11.467)

[44]Zhenyu Luo, Lihua Luo, Yichao Lu, Chunqi Zhu, Bing Qin, Mengshi Jiang, Xiang Li, Yingying Shi, Junlei Zhang, Yu Liu, Xinyu Shan, Hang Yin, Guannan Guan, Yongzhong Du, Ningtao Cheng, Jian You*, Dual-binding nanoparticles improve the killing effect of T cells on solid tumor. Journal of Nanobiotechnology 2022, 20, 261.  (IF: 9.429)

[45]Liu, Xu; Liu, Yu; Li, Xiang; Huang, Jiaxin; Guo, Xuemeng; Zhang, Junlei; Luo, Zhenyu; Shi, Yingying; Jiang, Mengshi; Qin, Bing; Du, Yong-Zhong; Luo, Lihua; You, Jian*, ER-targeting PDT converts tumor into in-situ therapeutic tumor vaccine, ACS nano 2022, 16, 9240-9250.  (IF: 18.027)

[46]Yingying Shi, Zhenyu Luo, Jian You*. Subcellular delivery of lipid nanoparticles to endoplasmic reticulum and mitochondria, WIREs Nanomedicine & Nanobiotechnology 2022, e1803. (IF: 9.42) 

[47]Yingying Shi, Yu Liu, Jiaxin Huang, Zhenyu Luo, Xuemeng Guo, Mengshi Jiang, Xiang Li, Yichao Lu, Xu Liu, Xinyu Shan, Lihua Luo, Jian You*. Optimized mobilization of MHC class I- and II- restricted immunity by dendritic cell vaccine potentiates cancer therapy. Theranostics 2022, 12, 3488-3502.(IF: 11.6)

[48]Yichao Lu, Yingying Shi, Zhenyu Luo, Xuemeng Guo, Mengshi Jiang, Xiang Li, Junlei Zhang, Chunqi Zhu, Hang Yin, Bing Qin, Xu Liu, Jiaxin Huang, Yongzhong Du, Lihua Luo, Jian You*. Reactivation of dysfunctional dendritic cells by a stress-relieving nanosystem resets anti-tumor immune landscape. Nano Today 2022, 43, 101416 (IF: 18.962)

[49]Chunqi Zhu, Yingying Shi, Qingpo Li, Lihua Luo, Xiang Li, Zhenyu Luo, Yichao Lu, Junlei Zhang, Mengshi Jiang, Bing Qin, Weigen Qiu, Zhaolei Jin, Xindong Jiang, Jiandong Xiao and Jian You*, Rational administration sequencing of immunochemotherapy elicits powerful anti-tumor effect. J Control Release 2022, 341, 769-781. (IF: 11.467)

[50]Bing Qin; Mengshi Jiang; Xiang Li; Yingying Shi; Junlei Zhang; Zhenyu Luo; Lihua Luo; Yichao Lu; Xu Liu; Sijie Wang; Yongzhong Du; Qingyun Qiu; Yan Lou; Jian You*. Oxygen Nanocarrier Broke the Hypoxia trap of Solid Tumors and Rescued Transfection Efficiency for Gene Therapy. Journal of Nanobiotechnology 2021, 19, 427.  (IF: 9.429)

[51]Yichao Lu, Yingying Shi, Jian You*. Strategy and clinical application of up-regulating cross presentation by DCs in anti-tumor therapy. J Control Release 2021, 341, 185-205. (IF: 11.467)

[52]Xindong Jiang, Tianxiang Shen, Zhaolei Jinyiji, Chunlong Li, Qingpo Li, Weigen Qiu, Yannan Cui, Zhihui Han, Xuemei Hou*, Jian You*. An analysis of the biopharmaceutical behaviour of proton pump inhibitors with different physicochemical properties.  Life Sciences 2021, 286, 120042. (IF: 6.78)

[53]Shi, Yingying; Lu, Yichao; Qin, Bing; Jiang, Mengshi; Guo, Xuemeng; Yin, Hang; Li, Xiang; Liu, Yu; Huang, Jiaxin; Liu, Huihui; Guan, Guannan; Peng, Ling; You, Jian*. Antigen transfer from non-APCs to APCs impacts the efficacy and safety of protein- and mRNA- based vaccines. Nano Today 2021, 41, 101326 (IF: 18.962)

[54]Lihua Luo; Bing Qin; Mengshi Jiang; Lin Xie; Zhenyu Luo; Xuemeng Guo; Junlei Zhang; Xiang Li; Chunqi Zhu; Yongzhong Du; Ling Peng; Jian You*. Regulating Immune Memory and Reversing Tumor Thermotolerance through a Step-by-Step Starving-Photothermal therapy. Journal of Nanobiotechnology 2021, 19, 297. (IF: 9.429)

[55]Jiang, Mengshi; Li, Xiang; Zhang, Junlei; Lu, Yichao; Shi, Yingying; Zhu, Chunqi; Liu, Yu; Qin, Bing; Luo, Zhenyu; Du, Yongzhong; Luo, Lihua; Peng, Ling; You, Jian*. Dual Inhibition of Endoplasmic Reticulum Stress and Oxidation Stress Manipulates the Polarization of Macrophages under Hypoxia to Sensitize Anti-PD-1 Immunotherapy. ACS Nano 2021, 15, 14522-14534. (IF: 18.027)

[56]Mengshi Jiang, Bing Qin, Lihua Luo, Xiang Li, Yingying Shi, Junlei Zhang, Zhenyu Luo,Chunqi Zhu, Guannan Guan, Yongzhong Du, Jian You*, A clinically acceptable strategy for sensitizing anti-PD-1 treatment by hypoxia relief. J Control Release 2021, 335, 408-419. (IF: 11.467)

[57]Xiang Li, Lihua Luo, Mengshi Jiang, Chunqi Zhu, Yingying Shi, Junlei Zhang, Zhenyu Luo, Xuemeng Guo, Bing Qin, Yichao Lu, Xinyu Shan, Yu Liu, Yongzhong Du, Ling Peng*, Jian You*. Cocktail strategy for ‘cold’ tumors therapy via active recruitment of CD8+ T cells and enhancing their function. J Control Release 2021, 334, 413-426. (IF: 11.467)

[58]Yingying Shi, Yichao Lu, Jian You*. Unfolded Protein Response in the Activation-Induced Biological Processes of CD8+ T Cells. Pharmacological Research 2021, 169, 105654. (IF: 10.334)

[59]Yingying Shi, Yichao Lu, Chunqi Zhu, Zhenyu Luo, Xiang Li, Yu Liu, Mengshi Jiang, Xu Liu, Lihua Luo, Yongzhong Du, Jian You*. Targeted regulation of lymphocytic ER stress response with an overall immunosuppression to alleviate allograft rejection. Biomaterials 2021, 272, 120757. (IF: 15.304)

[60]Chunqi Zhu, Yingying Shi, Jian You*. Immune cell connection by tunneling nanotubes: the impact of intercellular crosstalk on the immune response and its therapeutic applications. Molecular Pharmaceutics 2021, 18, 772-786. (IF: 5.364)

[61]Lihua Luo, Xiang Li, Junlei Zhang, Chunqi Zhu, Mengshi Jiang, Zhenyu Luo, Bing Qin, Yanqing Wang, Bin Chen, Yongzhong Du, Yan Lou, Jian You*. Enhanced immune memory through a constant Photothermal-Metabolism regulation for Cancer Prevention and Treatment. Biomaterials 2021, 270, 120678. (IF: 15.304)

[62]Yingying Shi, Chunqi Zhu, Yu Liu, Yichao Lu, Xiang Li, Bing Qin, Zhenyu Luo, Lihua Luo, Mengshi Jiang, Junlei Zhang, Guannan Guan, Cheng Zheng, Jian You*. A Vaccination with Boosted Cross Presentation by ER-Targeted Antigen Delivery for Anti-Tumor Immunotherapy.  Advanced Healthcare Materials 2021, 2001934. (IF: 11.092)

[63]Yingying Shi, Sijie Wang, Jialu Wu, Xizhi Jin, Jian You*, Pharmaceutical strategies for endoplasmic reticulum-targeting and their prospects of application. J Control Release 2021, 329, 337-352. (IF: 11.467)

[64]Mengshi Jiang, WeiLi, Chunqi Zhu, Xiang Li, Junlei Zhang, Zhenyu Luo, Bing Qin, Yongzhong Du, Lihua Luo, Jian You*.  Perdurable PD-1 blockage awakes anti-tumor immunity suppressed by precise chemotherapy. J Control Release 2021, 329, 1023-1036. (IF: 11.467)

[65]Bing Qin, Xiaoling Yuan, Mengshi Jiang, Hang Yin, Zhenyu Luo, Chunqi Zhu, Xiang Li, Junlei Zhang, Yingying Shi, Lihua Luo, Yongzhong Du, Jian You*. Targeting DNA to endoplasmic reticulum efficiently enhances gene delivery and therapy. Nanoscale 2020, 12, 18249-18262. （IF: 8.307）

[66]Hang Yin, Xiaoling Yuan, Lihua Luo, Yichao Lu, Junlei Zhang, Xinyu Shan, Chunqi Zhu, Jie Yang, Xiang Li, Mengshi Jiang, Bing Qin, Zhenyu Luo, Yingying Shi, Jian You*. Appropriate Delivery of the CRISPR/Cas9 System through the Nonlysosomal Route: Application for Therapeutic Gene Editing, Advanced Science 2020, 1903381.  (IF: 17.521)

[67]Xiaoling Yuan, Bing Qin, Hang Yin, Yingying Shi, Mengshi Jiang, Lihua Luo, Zhengyu Luo, Junlei Zhang, Xiang Li, Chunqi Zhu, Yongzhong Du, Jian You*. Virus-like Nonvirus Cationic Liposome for Efficient Gene Delivery via Endoplasmic Reticulum Pathway. ACS Central Science 2020, 6, 174-188. (IF: 18.728)

[68]Chunqi Zhu, Lihua Luo, Xindong jiang, Mengshi Jiang, Zhenyu Luo, Xiang Li, Weigen Qiu, Zhaolei Jin, Tianxiang Shen, Chunlong Li, Qingpo Li, Yunqing Qiu, Jian You*. Selective Intratumoral Drug Release and Simultaneous Inhibition of Oxidative Stress by a Highly Reductive Nanosystem and Its Application as an Anti-tumor Agent. Theranostics 2020, 10, 1166-1180.(IF: 11.6)

[69]Tianxiang Shen, Xindong Jiang, Zhaolei Jin, Qiuzhi Ji, Chunlong Li, Qingpo Li, Hongxin Long, Weigen Qiu, Wei Wang, Xuemei Hou*, Jian You*. The study of intestinal absorption and biodistribution in vivo of proton pump inhibitors. European Journal of Pharmaceutics and Biopharmaceutics 2020, 149, 135-144. (IF: 5.589)

[70]Chunqi Zhu, Xiaomeng Guo, Lihua Luo, Zhe Wu, Zhenyu Luo, Mengshi Jiang, Junlei Zhang, Bing Qin, Yingying Shi, Yan Lou,* Yunqing Qiu,* and Jian You*. Extremely Effective Chemoradiotherapy by Inducing Immunogenic Cell Death and Radio-Triggered Drug Release under Hypoxia Alleviation. ACS Appl. Mater. Interfaces 2019, 11, 46536-46547.  (IF: 10.383)

[71]Meng-Shi Jiang, Xiao-Yi Yin, Bing Qin, Shao-Yan Xuan, Xiao-Ling Yuan, Hang Yin, Chunqi Zhu, Xiang Li, Jie Yang, Yong-Zhong Du, Li-Hua Luo, and Jian You*, Inhibiting Hypoxia and Chemotherapy-Induced Cancer Cell Metastasis under a Valid Therapeutic Effect by an Assistance of Biomimetic Oxygen Delivery. Molecular Pharmaceutics 2019, 16, 4530-4541.(IF: 5.364)

[72]Wei Li, Jie Yang, Lihua Luo, Mengshi Jiang, Bing Qin, Hang Yin, Chunqi Zhu, Xiaoling Yuan, Junlei Zhang, Zhenyu Luo, Yong-Zhong Du, Qingpo Li  Yan Lou, Yunqing Qiu, Jian You*.Targeting photodynamic and photothermal therapy to the endoplasmic reticulum enhances immunogenic cancer cell death. Nature Communications 2019, 10, 3349. (IF: 17.694)

[73]Jing Qi, Weishuo Li, Kongjun Lu, Feiyang Jin, Di Liu, Xiaoling Xu, Xiaojuan Wang, Xuqi Kang,  Wei Wang, Gaofeng Shu, Feng Han, Xiaoying Ying, Jian You*  Jiansong Ji* Yongzhong Du*.pH and Thermal Dual-Sensitive Nanoparticle-Mediated Synergistic Antitumor Effect of Immunotherapy and Microwave Thermotherapy. Nano Letters 2019, 19, 499-4959.(IF: 12.262)

[74]Jie Yang, Wei Li, Lihua Luo, Mengshi Jiang, Chunqi Zhu, Bing Qin, Hang yin, Xiaoling Yuan, Yongzhong Du, Jian You*. Hypoxic tumor therapy by hemoglobin-mediated drug delivery and reversal of hypoxia-induced chemoresistance. Biomaterials 2018, 182, 145-156.(IF: 15.304)

[75]Lihua Luo, Chunqi Zhu, Hang Yin, Mengshi Jiang, Junlei Zhang, Bing Qin, Zhenyu Luo, Xiaoling Yuan, Jie Yang, Wei Li, Yongzhong Du, Jian You*. Laser Immunotherapy in Combination with Perdurable PD-1 Blocking for Treatment of Metastatic Tumor. ACS nano 2018, 12, 7647-7662. (IF: 18.027)

[76]Lihua Luo, Chunqi Zhu, Jie Yang, XiaomengGuo, Wei Li, Xiaoyi Yin, Mengshi Jiang, Bing Qin, Xiaoling Yuan, Hang Yin, Qingpo Li, Yongzhong Du, Jian You*, Perdurable Anti-PD-1 Immunotherapy Together with Photothermal Ablation against Malignant and Distant Tumors. J Control Release 2018, 278, 87-99.(IF: 11.467)

[77]Qingpo Li, Wei Li, Haixiao Di, Lihua Luo, Chunqi Zhu, Jie Yang, Xiaoyi Yin, Hang yin, Jianqing Gao, Yongzhong Du, Jian You*. A photosensitive liposome with NIR light triggered doxorubicin release as a combined photodynamic-chemo therapy system. J Control Release 2018, 277, 114-125. (IF: 11.467)

[78]Chunqi Zhu, Hanbo Zhang, Wei Li, Lihua Luo, Xiaomeng Guo, Zuhua Wang, Fenfen Kong, Qingpo Li, Jie Yang, Yongzhong Du, Jian You*. Suppress orthotopic colon cancer and its metastasis through exact targeting and highly selective drug release by a smart nanomicelle. Biomaterials 2018, 161, 144-153.(IF: 15.304)

[79]Wei Li, Wanqing Hou, Xiaomeng Guo, Lihua Luo, Qingpo Li, Chunqi Zhu, Jie Yang, Jiang Zhu, Yongzhong Du, Jian You*. A temperature-controlled and phase-transition ultrasound imaging-guided photothermal-chemotherapy triggered by NIR light. Theranostics 2018, 8, 3059-3073. (IF: 11.6)

[80]Xiaomeng Guo, Jiaxin Qu, Wei Li, Lihua Luo, Chunqi Zhu, Jie Yang, Xiaoyi Yin, Qingpo Li, Yongzhong Du, Dawei Chen, Jian You*. Synchronous delivery of oxygen and photosensitizer for alleviation of hypoxia tumor microenvironment and dramatically enhanced photodynamic therapy. Drug Delivery 2018, 25. 585-599. (IF: 6.819)

[81]Zuhua Wang, Shaoyan Xuan, Wenqi Qiu, Jiang Zhu, Xiaomeng Guo, Wei Li, Hanbo Zhang, Xiuliang Zhu, Yong-Zhong Du, Jian You*. Near infrared light mediated photochemotherapy for efficiently treating deep orthotopic tumors guided by ultrasound imaging. Drug Delivery 2017, 24, 1441-1452. (IF: 6.819)

[82]Jiaxin Qu, Xiaomeng Guo, Wei Li, Wanqing Hou, Hanbo Zhang, Lihua Luo, Chunqi Zhu, Jie Yang, Xiaoyi Yin, Yuan Tao, Yongzhong Du, Yan Lou, Dawei Chen, Jian You*. Preparation of artificial red cell and its application on alleviation of tumor hypoxia. Colloids and Surfaces B: Biointerfaces 2017, 160, 446-454. (IF: 5.999)

[83]Hanbo Zhang, Wei Li, Xiaomeng Guo, Fenfen Kong, Zuhua Wang, Chunqi Zhu, Lihua Luo, Qingpo Li, Jie Yang, Yongzhong Du, Jian You*. Specifically Increased Paclitaxel Release in Tumor and Synergetic Therapy by a Hyaluronic Acid-Tocopherol Nanomicelle. ACS Appl. Mater. Interfaces 2017, 9, 20385-20398. (IF: 10.383)

[84]Wei Li, Xiaomeng Guo, Fenfen Kong, Hanbo Zhang, Lihua Luo, Qingpo Li, Chunqi Zhu, Jie Yang, Yongzhong Du, Jian You*. Overcoming photodynamic resistance and tumor targeting dual-therapy mediated by indocyanine green conjugated gold nanospheres. J Control Release 2017, 258, 171-181. (IF: 11.467)

[85]Xiaomeng Guo, Wei Li, Lihua Luo, Zuhua Wang, Qingpo Li, Fenfen Kong, Hanbo Zhang, Jie Yang, Chunqi Zhu, Yongzhong Du, and Jian You*. External Magnetic Field-Enhanced Chemo-Photothermal Combination Tumor Therapy via Iron Oxide Nanoparticles. ACS Appl. Mater. Interfaces 2017, 9, 16581–16593. (IF: 10.383)

[86]Xiaomeng Guo, Wei Li, Jialin Zhou, Wanqing Hou, Xue Wen, Hanbo Zhang, Fenfen Kong, Lihua Luo, Qingpo Li, Yongzhong Du, and Jian You*. Specific Photothermal Ablation Therapy of Endometriosis by Targeting Delivery of Gold Nanospheres. Small 2017, 13, 1603270. (IF: 15.153)

[87]Xiaomeng Guo, Jian You*. Near infrared light-controlled therapeutic molecules release of nanocarriers in cancer therapy. Journal of Pharmaceutical Investigation. 2017, 47, 297-316.

[88]Fei Liu, Fenfen Kong, Qingpo Li, Hong Yuan, Yongzhong Du, Fuqiang Hu, Jihong Sun, Jian You*. Low MW polyethylenimine conjugated gold nanospheres: a platform for selective genetherapy controlled by NIR light. Nanomedicine-UK 2017, 12, 511-534. (IF: 6.096)

[89]Wei Li, Hanbo Zhang, Xiaomeng Guo, Zuhua Wang, Fenfen Kong, Lihua Luo, Qingpo Li, Chunqi Zhu, Jie Yang, Yan Lou, Yongzhong Du, Jian You*. Gold Nanospheres-Stabilized Indocyanine Green as a Synchronous Photodynamic-Photothermal Therapy Platform That Inhibits Tumor Growth and Metastasis. ACS Appl. Mater. Interfaces 2017, 9, 3354-3367. (IF: 11.383)

[90]Nianxi Zhao, Jian You, Zihua Zeng, Chun Li, Youli Zu. An Ultra pH-Sensitive and Aptamer-Equipped Nanoscale Drug-Delivery System for Selective Killing of Tumor Cells. Small, 2013, 

[91]Jian You, Zuhua Wang, Yongzhong Du, Hong Yuan, Peizun Zhang, Jialin Zhou, Fei Liu, Chun Li, and Fuqiang Hu. Specific tumor delivery of paclitaxel using glycolipid-like polymer micelles containing gold nanospheres. Biomaterials, 2013, 34, 4510-4519. 

[92]Jian You, Rui Zhang, Chiyi Xiong, Meng Zhong, Maritess Melancon, Sanjay Gupta, Alpa M. Nick, Anil K. Sood, Chun Li, Effective Photothermal Chemotherapy Using Doxorubicin-Loaded Gold Nanospheres that Target EphB4 Receptors in Tumors, Cancer Res; 2012, 72(18), 4777-4786. 

[93]Zhang G, Zhang R, Melancon MP, Wong K, You J, Huang Q, Bankson J, Liang D, Li C. The degradation and clearance of Poly(N-hydroxypropyl-l-glutamine)-DTPA-Gd as a blood pool MRI contrast agent. Biomaterials, 2012 , 33, 5376-83. 

[94]Yongzhong Du, Lili Cai, Ping Liu, Jian You, Hong Yuan, Fuqiang Hu, Tumor cells-specific targeting delivery achieved by A54 peptide functionalized polymeric micelles, Biomaterials, 2012, 33, 85858-8867. 

[95]Hong Yuan*, Xin Bao, Yong-Zhong Du, Jian You, Fu-Qiang Hu*. Preparation and evaluation of SiO2-deposited stearic acid-g-chitosan nanoparticles for Doxorubicin delivery. Int J Nanomed. 2012, 7, 5119-5118.

[96]Fuqiang Hu, Yinying Zhang, Jian You, Hong Yuan, Yongzhong Du, pH Triggered Doxorubicin Delivery of PEGylated Glycolipid Conjugate Micelles for Tumor Targeting Therapy, Mol. Pharmaceutics, 2012, 9, 2469–2478. 

[97]Sanjay Gupta, Jason Stafford, Sanaz Javadi, Efe Ozkan, Joe E. Ensor, Kenneth C. Wright, Andrew M. Elliot, You Jian, Rita E. Serda, Katherine A. Dixon, Jennifer J. Miller, Sherry Klump, DVM, Michael J. Wallace, Chun Li, Effects of Near-infrared Laser Irradiation of Biodegradable Microspheres Containing Hollow Gold Nanospheres and Paclitaxel Administered Intraarterially in a Rabbit Liver Tumor Model, J Vasc Interv Radiol, 23, 553-561, 2012. 

[98]Haifa Shen, Jian You (Co-first author), Guodong Zhang, Arturas Ziemys, Qingpo Li, Litao Bai, Xiaoyong Deng, Donald R. Erm, Xuewu Liu, Chun Li, Mauro Ferrari, Cooperative, Nanoparticle-Enabled Thermal Therapy of Breast Cancer, Adv Healthcare Mater, 2012, 1, 84-89. 

[99]Sanjay Gupta, Sanaz Javedi, Efe Ozkan, You Jian, Ken Wright, Chun Li, Michael Wallace. Laser activated release of paclitaxel from biodegradable microspheres containing hollow gold nanospheres and paclitaxel administered intra-arterially into liver tumor in an animal model. J Vasc Interv Radiol. 2011. 22, S11. 

[100]Jian You, Rui Zhang, Guodong Zhang, Meng Zhong, Yang Liu, Carolyn S. Van Pelt, Dong Liang, Wei Wei, Anil K. Sood, Chun Li. Photothermal-chemotherapy with doxorubicin-loaded hollow gold nanospheres: A platform for near infrared light-trigged drug release. J Control Release. 2012, 158, 319-328. 

[101]Jian You, Guodong Zhang, Chun Li. Exceptionally High Payload of Doxorubicin in Hollow Gold Nanospheres for near-Infrared Light-Triggered Drug Release. ACS nano, 2010, 4 (2), 1033-1041. 

[102]Jian You, Ruping Shao, Xin Wei, Sanjay Gupta, Chun Li. Near-Infrared Light Triggers Release of Paclitaxel from Biodegradable Microspheres: Photothermal Effect and Enhanced Antitumor Activity. Small, 2010, 6 (9), 1022-1031. 

[103]Jian You, Fuqiang Hu, Yongzhong Du, Hong Yuan. Improved cytotoxicity of doxorubicin by enhancing its nuclear delivery mediated via nanosized micelles. Nanotechnology. 2008, 19 (25), 255103. 

[104]Jian You, Xin Li, Fude Cui, Yongzhong Du, Hong Yuan, Fuqiang Hu. Folate conjugated polymer micelles for active-targeting to cancer cells: preparation, in vitro evaluation of targeting ability and cell cytotoxicity. Nanotechnology, 2008, 19 (4), 511-519. 

[105]Feng Wan, Jian You, Yu Sun, Fude Cui, Yongzhong Du, Fuqiang Hu. Studies on PEG modified SLNs loading Vinorelbine Bitartrate (1): Preparation and evaluation in vitro. Int J Pharm, 2008, 359 (1-2), 104-110. 

[106]Fuqiang Hu, Xiuling Wu, Yongzhong Du, Jian You, Hong Yuan. Cellular uptake and cytotoxicity of shell crosslinked stearic acid-grafted chitosan oligosaccharide micelles encapsulating doxorubicin. Eur J Pharm Biopharm. 2008, 69 (1), 117-125. 

[107]Fuqiang Hua, Pan Meng, Youqin Dai, Yongzhong Du, Jian You, Xiaohong Wei, Hong Yuan. PEGylated chitosan-based polymer micelle as an intracellular delivery carrier for anti-tumor targeting therapy. Eur J Pharm Biopharm. 2008, 70 (3), 749-757. 

[108]Hong Yuan, Jing Miao, Yongzhong Du, Jian You, Fuqiang Hu, Su Zeng. Cellular uptake of solid lipid nanoparticles and cytotoxicity of encapsulated paclitaxel in A549 cancer cells. Int J Pharm, 2008, 348 (1-2), 137-145. 

[109]Hong Yuan, Lingfei Huang, Yongzhong Du, Xiaoying Ying, Jian You, Fuqiang Hu and Su Zeng. Solid lipid nanoparticles prepared by solvent diffusion method in a nanoreactor system. Colloid surface B, 2008, 61 (2), 132-137. 

[110]Jian You, Fuqiang Hu, Yongzhong Du, Hong Yuan. Polymeric micelles with glycolipid-like structure and multiple hydrophobic domains for mediating molecular-target delivery of paclitaxel. Biomacromolecules, 2007, 8 (8), 2450-2456. 

[111]Jian You, Fuqiang Hu, Yongzhong Du, Hong Yuan. High cytotoxicity and resistant-cell reversal of novel paclitaxel loaded micelles by enhancing molecular-target delivery of the drug. Nanotechnology, 2007, 18 (49), 511-517. 

[112]Jian You, Feng Wan, Fude Cui, Yu Sun, Fuqiang Hu, Yongzhong Du. Preparation and characteristic of vinorelbine bitartrate loaded solid lipid nanoparticles. Int J Pharm, 2007, 343 (1-2), 270-276. 

[113]Hong Yuan, Leilei Wang, Yongzhong Du, Jian You, Fuqiang Hu, Su Zeng. Preparation and characteristics of nanostructured lipid carriers for control-releasing progesterone by melt-emulsification. Colloid Surface B, 2007, 60 (2), 174-179. 

发表中文论文：

[1]李娜, 游剑. 载血卟啉单甲醚中空金纳米球的光热光动力联合抗肿瘤研究[J]. 中国现代应用药学, 2020, 37 (21): 2617-2624.

[2]孙晶晶, 席建军, 潘旭旺, 邵益丹, 方红英, 潘金明, 张建康, 庄让笑, 游剑. 新型抗肝损伤化合物XXH-32脂质体的制备及其药剂学性质研究[J]. 中国现代应用药学, 2019, 36 (04): 411-416.

[3]章瑾, 谢明华, 蔡鑫君, 游剑. 自乳化给药系统对雷公藤甲素的减毒作用研究[J]. 中国现代应用药学, 2019, 36 (02): 168-171.

[4]侯婉晴, 郭晓萌, 罗利华, 范玉玲, 游剑. 一种纳米级超声造影剂的制备及其性质考察[J]. 中国药剂学杂志(网络版), 2017, 15 (05): 111-118.

[5]侯婉晴, 郭晓萌, 罗利华, 范玉玲, 游剑. 近红外光介导的尺寸可逆转变超声造影剂的制备及其性质[J]. 药学学报, 2017, 52 (09): 1458-1465.

[6]张惠檄, 游剑. 3D打印阿司匹林速释缓释双层片[J]. 中国药学杂志, 2017, 52 (04): 298-302.

[7]刘萍, 翁旗, 游剑, 袁弘, 胡富强, 杜永忠. 基于疏水改性葡聚糖胶束药物传递系统的研究[A] 2012年中国药学大会暨第十二届中国药师周论文集[C]. 中国药学会、江苏省人民政府, 中国药学会, 2012: 10.

[8]游剑. 探讨新学科在药物制剂教学中的引入[J]. 海峡药学, 2012, 24 (08): 260-262.

[9]胡富强. 泮托拉唑钠制剂关键技术研究及产业化. 浙江省, 浙江大学, 2010-05-01.

[10]应晓英, 徐晖, 游剑, 许乐幸. 乳化剂HLB值对莪术油亚微乳理化性质的影响[J]. 中国现代应用药学, 2009, 26 (03): 227-230.

[11]袁弘. 抗肿瘤靶向脂质纳米给药系统的制备与应用. 浙江省, 浙江大学, 2008-07-13.

[12]高建青, 成红, 应晓英, 游剑. 在教学中培养学生解决实际问题的能力[J]. 药学教育, 2008, (02): 28-30.

[13]胡富强. 细胞浆靶向壳寡糖-硬脂酸嫁接物纳米载体材料的制备与应用. 浙江省, 浙江大学药学院, 2007-02-01.

[14]李青坡, 游剑, 王永圣, 赵颖, 杨蕾, 崔福德. 葛根芩连微丸中指标成分的体外同步释放研究[J]. 中草药, 2006, (01): 40-44.

[15]李青坡,游剑,杨蕾,赵颖,王永圣,崔福德. 挤出滚圆法制备葛根芩连微丸[J]. 中草药, 2005, (10): 37-40.

[16]游剑. 乳化溶剂扩散法制备具有自乳化功能莪术油微球的研究及体内评价[D]. 沈阳药科大学, 2005.

[17]游剑,李青坡,于英伟,崔福德. 应用HPLC研究莪术油的药动学[J]. 中国药学杂志, 2005, (05): 63-65.

[18]游剑,于英伟,李青坡,崔福德. 莪术油微球的含量测定[J]. 中成药, 2005, (01): 29-32.

[19]游剑,李青坡,于英伟,崔福德. 运用单向灌流模型研究莪术油中三成分对大鼠在体肠的吸收[J]. 药学学报, 2004, (10): 849-853.

[20]李青坡,王永圣, 游剑 ,崔福德. 葛根总异黄酮提取工艺的研究[J]. 中国药业, 2004, (05): 46-47.

会议论文：

[1]游剑. (2014). 近红外光介导的药物传递与光热治疗(英文). (eds.) 2014中国药物制剂大会——中国药学会药剂专业委员会年会、第六届亚洲阿登制药技术研讨会暨国际控释协会中国分会年会会议论文集 (pp.200+199).  

[2]刘萍, 翁旗, 游剑, 袁弘, 胡富强 &  杜永忠. (2012). 基于疏水改性葡聚糖胶束药物传递系统的研究. (eds.) 2012年中国药学大会暨第十二届中国药师周论文集 (pp.1171-1180). 

[3]李云龙, 游剑, 杜永忠, 袁弘, 胡富强 &  虞和永. (2012). The study on paclitaxel loaded lipid nanoparticles to reverse tumor resistance. (eds.) 2012年中国药物制剂大会——中国药学会药剂专业委员会年会、亚洲阿登制药技术研讨会暨国际控释协会中国分会年会会议资料 (pp.344-345).

先后获“沈阳药科大学优秀博士论文”和“辽宁省优秀博士论文”。 

1. 浙江大学求是特聘教授，2023年

2. 国家万人计划，2020年

3. 科技部中青年科技创新领军人才，2019年

4. 浙江省科技进步一等奖，2017年

5. 浙江省医药卫生科技奖一等奖，2015年

6. 浙江省科技进步一等奖，2014年

7. 浙江省科技进步三等奖，2007年

8. 教育部自然科学奖二等奖，2009年

9. 中国药学会青年药剂学奖，2012年

实验室致力于产学研的结合，团队由基础研究团队和专业临床转化团队组成。基础研究团队拥有硕士、博士生以及博士后20余名；专业创新药临床转化团队由8名工作人员组成（其中高级工程师4人），具有丰富的从基础研究到新药研发经验和经历。

基础研究团队：

博后及科研助理 

临床转化团队：

 

实验室近几年设备投入近2000万元，拥有先进的基础实验研究设备，以及创新药物临床转化实验研发平台，实施规范化、标准化研发管理模式。

实验室研发平台3大功能：

1）以显著性临床优势为目标，紧密学-研-产为手段的源头性创新制剂研发。

2）以制剂临床推广与更新换代升级为目标的已有制剂基础性研究。

3）以疗效与安全性为目标，将现有技术平台优势向不同制剂领域的辐射与拓展。

实验室设备：

光热消融与化疗相结合为肿瘤治疗带来新希望
——访浙江大学药学院副教授游剑 

游剑，药剂学博士，副教授，硕士生导师。2000年就读于沈阳药科大学药学院药剂专业，2005年获博士学位, 同年就职于浙江大学药学院，2007年晋升副教授，2008年至2011年在美国德克萨斯大学安德森癌症研究中心（University of Texas MD Anderson Cancer Center）做访问学者、博士后。主要致力于新型纳米给药系统的基础研究与应用基础研究工作，具体包括：（1）药物分子靶向纳米传递系统研究，即运用具有细胞浆或细胞核靶向分布功能的壳聚糖–硬脂酸聚合物纳米载体，实现对药物分子作用靶标的药物靶向传递，为高效安全药物治疗提供新的策略与方法；（2）纳米光热效应传递系统研究，即采用一种具有近红外光热转化功能的新型纳米材料中空纳米金，实现肿瘤靶向光热治疗。在此基础上，开创性地将抗肿瘤药物与中空金纳米粒结合，形成一种新型多模式肿瘤治疗纳米系统，产生肿瘤靶向光热消融治疗，药物光热控制释放以及肿瘤靶向化疗多重作用，成功解决肿瘤光热治疗中热量在肿瘤内部分布不均导致的肿瘤易于复发的问题，为肿瘤的有效治疗发展了新技术、新理论。研究成果以第一或通讯作者先后在 ACS nano、Cancer research、Small、Journal of controlled release、Biomacromolecules等期刊上发表，共发表SCI论文三十余篇，获国家发明专利授权15项。获国家教育系统自然科学二等奖1项，2012年获中国药学会–石药青年药剂学奖。 

游剑博士长期从事肿瘤光热消融治疗的研究，通过钴粒子还原金离子成功合成一种小粒径、单分散性、具有强近红外光热效应的金纳米材料（中空金纳米粒），并首次实现放大重复性生产。与目前具有类似功效，且在美国进入临床实验的金–硅壳核结构纳米粒（粒径100～150nm）相比，中空金纳米粒具有更小粒径（30～50nm）、更简单结构（无硅核）、更强的近红外光热效应（产生相同的近红外光热效果，中空金纳米粒的金材料用量是金–硅壳核结构纳米粒金用量的1/4～1/3），这预示中空金纳米粒在癌症光热消融治疗应用中可能具有更美好的前景。在裸鼠乳腺癌模型的治疗中，产生明显的肿瘤近红外光热消融作用。 

为克服单纯光热消融治疗缺陷以获得更高的肿瘤治疗效果，游剑博士将中空金纳米粒与抗肿瘤药物相结合，构建一种新型单一载体系统，呈现光热消融与化疗相结合的新型肿瘤多重模式治疗。光热效应产生消融作用的同时，可以实现药物实时控制释放，并与之产生的化疗作用相互促进，协同起效，极大提高了肿瘤有效治疗的可能性。具体为：（1）中空金纳米粒和药物（紫杉醇）共同包裹于聚合物形成的微球中，用于近红外光介导的家兔肝癌模型栓塞、光热消融治疗；（2）将表面改性的中空金纳米粒和药物包封与壳聚糖–硬脂酸胶束中，借助该胶束高效细胞摄取和亚细胞靶向功能实现癌症有效治疗；（3）利用中空金纳米粒表面电负性、中空性和多孔性，将药物（阿霉素）直接装于中空金纳米粒的内外表面，由于粒子比表面较大而呈现出极高的药物装载效率（最大药物含量可达60%w/w）。 

这种直接药物装载于粒子的方式优势为：粒子较易控制（主要受中空金纳米粒影响）以及易于进行表面修饰（利用Au-S键结合完成粒子修饰）。显著的抗肿瘤功效在裸鼠的乳腺癌和卵巢癌模型治疗中得到体现（治疗组给药24h后的近红外光一次照射，3周后一半动物的肿瘤完全消失，剩余老鼠肿瘤生长明显缓于单纯药物化疗对照组，且没有单纯化疗组表现出来的明显毒性情况）。当这种新型多模式治疗纳米系统采用一种配体（多肽与肿瘤表面过度表达的受体EphB4存在特异性结合）进行表面修饰后，该纳米系统可以进一步提高自身在肿瘤部位的累积（与未有配体的纳米粒子相比，提高2～4倍的肿瘤累积量），结果是进一步增加了治疗效果和降低了毒副作用。在较低的给药剂量以及安全的近红外光照条件下实现模型动物肿瘤的完全消除，肿瘤不存在复发现象。 

该项研究成果发表在今年的Cancer Research杂志上，并成为封面论文，受到广泛关注。游剑博士表示，接下来的工作会集中在纳米材料的大动物毒理研究方面，同时积极推动中空金纳米粒作为一种新型恶性肿瘤治疗药物进入临床实验研究，进一步运用该材料用于耐药肿瘤的攻克。如果该项研究成功，将会对肿瘤治疗产生革命性的推动作用，解决目前肿瘤治疗中放化疗低效高毒以及肿瘤耐药性等难以攻克的问题，显著提高癌症患者治愈率。

 《科技成果管理与研究》2013年05月27日